| EBSCO Information Services | Birmingham | 1943 |
| Eckert–Mauchly Computer Corporation | Filadelfia | 1948 |
| Ecko Unlimited | Municipio de East Brunswick | 1993 |
| Eclipse Comics | Forestville, Staten Island | 1977 |
| Edelman | Nueva York | 1952 |
| Edison Manufacturing Company | Nueva York | 1889 |
| Einstein Bros. Bagels | Lakewood | 1995 |
| Electro-Motive Diesel | La Grange | 1922 |
| Electronic Data Systems | Plano | 27 de junio de 1962 |
| Elevance Health | Indianápolis | 2004 |
| Elliott Investment Management | Nueva York | 1977 |
| Emerson Electric | San Luis | 24 de septiembre de 1890 |
| Encyclopædia Britannica | Chicago | 1768 |
| Endeavor Group Holdings | Beverly Hills | 1898 |
| Energy Transfer Partners | Dallas | 1996 |
| EnterpriseDB | Bedford | marzo 2004 |
| Epicor | Austin, Irvine | 1972 |
| Epyx | San Francisco | 1978 |
| ESPN+ | Estados Unidos | 12 de abril de 2018 |
| Ethyl Corporation | Richmond | 1921 |
| Euronet Worldwide | Leawood | 1994 |
| Evolve | Filadelfia | 2009 |
| Expedia Group | Seattle | 22 de octubre de 1996 |
| Expeditors International | Seattle | 1979 |
| Extreme Championship Wrestling | Filadelfia | 25 de abril de 1992 |
| Fageol | Oakland | 1916 |
| Fantasy Flight Games | Roseville | 1995 |
| Farmacéutica Vertex | Boston | 1989 |
| Farmers Insurance Group | Wilshire Boulevard, Los Ángeles | 1928 |
| Fastly | San Francisco | 2011 |
| Federal Home Loan Banks | Boston, Chicago, Pittsburgh, Dallas, Atlanta, Des Moines, Topeka, Cincinnati, Nueva York, San Francisco, Indianápolis | 1932 |
| FedEx | Memphis | 1971 |
| FedEx Office | Dallas | 1970 |
| FEI Company | Hillsboro | 1971 |
| Fender Musical Instruments Corporation | Nabari Station | 1946 |
| Fenty Beauty | 425 Market Street | 8 de septiembre de 2018 |
| Fenway Sports Group | Boston | 2001 |
| Ferrocarril del Pacífico Norte | Saint Paul | 2 de julio de 1864 |
| Fidelity Investments | Boston | 1946 |
| Firebase | San Francisco | 2010 |
| FireEye | Milpitas | febrero 2004 |
| Firehouse Subs | — | 1994 |
| First Citizens BancShares | Raleigh | 7 de agosto de 1986 |
| Fiserv | Brookfield | 1984 |
| Florsheim Shoes | Glendale | 1892 |
| Fluke Corporation | Everett | 1948 |
| Fluor Corporation | Irving | 1890 |
| FMC Technologies | Houston | 2000 |